Luminex MAGPIX User Quick Guide Uživatelský manuál

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Strany 1 - Guide 4.2

MAGPIX® User QuickGuide 4.2

Strany 2

System InitializationSystem initialization prepares the system for data acquisition. On initial startup, the SystemInitialization should consist of:•

Strany 3

1. Load the CAL/VER CD (provided with the kit) on the computer.2. On the Home page, click System Initialization.3. Click Import Kit at the bottom righ

Strany 4 - Table of Contents

Defining the System Initialization RoutineNOTE: Luminex recommends daily performance verification and weeklycalibration of the MAGPIX system. You can

Strany 5

3. Under Maintenance Options, select Fluidics prep, performance verification from thedrop-down list. FIGURE 2.Setting System Initialization Routine4.

Strany 6 - Start-up Indicators

Creating a Quantitative Assay ProtocolThe protocol must contain multiple standards. The standards are assigned lot valueinformation for each test. A s

Strany 7 - Initial Startup

15. In the Analysis Settings dialog box, select the analysis method from the Method list,and the weighting in the Weight Type list. Click Apply to All

Strany 8

27. Click Next. The Plate Layout tab opens. • To add well commands, highlight the appropriate wells and mark them as unknown,standard, control, backgr

Strany 9 - Revive After Storage Routine

default Post Batch routine can be changed in the Batch Optionstab of the Admin page.Creating a KitTo create a kit:1. Open the Protocols page, then ope

Strany 10 - System Initialization

Create a New Batch from an Existing ProtocolThis option creates a new batch using a selected protocol from the Installed Protocols list.The list conta

Strany 11

1. Open the Results page, then the Reports tab.2. In the Report drop-down list, select the category of report: batch, protocol, calibrationand verific

Strany 12

© Luminex Corporation, 2011. All rights reserved. No part of this publication may bereproduced, transmitted, transcribed, or translated into any langu

Strany 13

Shutting Down the AnalyzerRun the daily shutdown routine to prevent clogs and crystallization of salt in the sampleprobe. Clogs and crystallization of

Strany 14 - Chapter 3: Running Assays

MAGPIX® User Quick Guide 4.216

Strany 15

If you are not successful troubleshooting problems with MAGPIX, contact Luminex TechnicalSupport. See Technical Support. If necessary, attach a suppor

Strany 16

FIGURE 3.MAGPIX Off-Plate Reagent Block1 Reservoirs2 Strip WellsMAGPIX New System Startup ErrorsProblem Resolution1. Check the sample probe height for

Strany 17 - Creating a Lot

Problem Resolution(1x) Clean with 0.1 N NaOH (reservoir C)(3x) Rinse (reservoir D)Run Calibration routine. Verify that reagent is freshly vortexed. Us

Strany 18 - Generating a Report

Problem ResolutionVER (strip well B)Verify that reagent is freshly vortexed. Use 6 drops of reagent.(1x) Rinse (reservoir D)Fluidics 1 (strip well C)V

Strany 19

MAGPIX Calibration and Performance VerificationTroubleshooting GuideTroubleshooting21

Strany 20 - Chapter 4: Shutdown

TABLE 1.Fittings to CheckFitting Location, Step, andFittingFluid CompartmentStep 3: Drive Fluid FittingFluid CompartmentStep 4: Waste ContainerFitting

Strany 21 - User Quick Guide 4.2

You can access the Technical Support website using a user name and password athttps://oraweb.luminexcorp.com/OA_HTML/jtflogin.jsp.By Phone Inside the

Strany 22 - Chapter 5: Troubleshooting

8. Verify the location where you want to store the file. To change the location, click Browse,then navigate to the new folder and click OK.9. Click Sa

Strany 23 - FIGURE 3

MAGPIX® User Quick Guide 4.2ii

Strany 24 - Troubleshooting

Table of ContentsChapter 1 Starting the MAGPIX System for the First Time ...1Starting xPONENT ...

Strany 25 - Changing Sample Load Volume

MAGPIX® User Quick Guide 4.2iv

Strany 26 - Troubleshooting Guide

Detailed instructions on how to install the MAGPIX System are provided in the PC InstallationInstructions (89-30000-00-263) and Installing MAGPIX (89-

Strany 27 - Technical Support

• Blue light indicator turns on.• Fans turn on.• Syringe pump initializes.• The filter wheel initializes.• Software connects to the instrument.NOTE: I

Strany 28 - Sending a Support.zip File

1. On the Home page, click Probe and Heater under Daily Activities. The Probe &Heater tab opens.2. Use well D6 (this is the center of a standard 9

Strany 29

FIGURE 1.Sample Probe Height AdjustmentRevive After Storage RoutineNOTE: The Revive After Storage routine is necessary when the systemruns for the fir

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