Luminex 200 User Manual with LDS 1.7 Software Uživatelský manuál

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Strany 1 - LDS Version 1.7

Luminex 200™ User Manual for LDS Version 1.7 Lum/nex ®

Strany 2

iv PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologyBead Detail Irregularities . . . . . . . . . . . . . . . . . .

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 12 PN 89-00002-00-150 Rev. A

Strany 4

Luminex 200 Maintenance Log Month(s): ________________________________________Year:_______________Procedure: To use this lo

Strany 6 - EULA PN: 89-30000-00-070

PN 89-00002-00-150 Rev. A 8 - 1TroubleshootingTroubleshooting procedures help users isolate, identify, and remedy problems with the Luminex analyzer a

Strany 7 - Contents

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 2 PN 89-00002-00-150 Rev. AFor assistance, contact Luminex Technical Support via email.

Strany 8 - Data Acquisition 6-1

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 3• Instrument control, sample acquisition, session uploading, and start, stop and pause f

Strany 9 - Troubleshooting 8-1

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 4 PN 89-00002-00-150 Rev. AFluid Leaks Fluid leaks can result in poor pressurization an

Strany 10 - Product Numbers B-1

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 5Sample Probe Problems with the sample probe can lead to fluid leaks and pressurization p

Strany 11 - Index Index-1

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 6 PN 89-00002-00-150 Rev. ASample arm stuck in the down positionThe sample probe height

Strany 12

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 7Calibration ProblemsThe sample probe is bent. Remove the sample probe from the Luminex a

Strany 13 - Introduction

PN 89-00002-00-150 Rev. A vxMAP Technology ContentsLuminex Sheath Delivery System C-1Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Strany 14

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 8 PN 89-00002-00-150 Rev. AThe calibration microspheres are not fully suspended.Vortex

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xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 9Acquisition ProblemsBeads were left in the system after Classification calibration.Perfo

Strany 16 - Measurements

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 10 PN 89-00002-00-150 Rev. AThe wrong wells are selected in the Setup XY tab.On the Opt

Strany 17 - Assistance

xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 11Bead Detail Irregularities You can diagnose many problems by using the histogram descri

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 12 PN 89-00002-00-150 Rev. ACalibration microspheres classify too highYou may be using

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xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 13Error MessagesError Type Possible Cause SolutionUnable to calculate temperature compens

Strany 20 - Regulatory Symbols

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 14 PN 89-00002-00-150 Rev. APrinting ErrorsVerificationPerform the verification procedu

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xMAP Technology TroubleshootingPN 89-00002-00-150 Rev. A 8 - 151. Click Warmup on the toolbar. Wait 30 minutes for the warmup to complete.2. On the An

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology8 - 16 PN 89-00002-00-150 Rev. Afrom the Luminex 200 system, copy the files to a floppy dis

Strany 23

PN 89-00002-00-150 Rev. A 9 - 1ProtocolsUpdates or additions to these protocols are posted on the Luminex website at http://www.luminexcorp.com.The pr

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vi PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

Strany 25 - Biological

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 2 PN 89-00002-00-150 Rev. A3. Sonicate the vial in a water bath sonicator to disperse t

Strany 26 - Blue Indicator Light

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 3Enumeration of xMAP Microsphere SuspensionsLuminex xMAP microspheres are provided at standard

Strany 27 - System Overview

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 4 PN 89-00002-00-150 Rev. AxMAP Microsphere Stability and StoragexMAP microspheres are

Strany 28

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 5• Sulfo-NHS: N-Hydroxysulfosuccinimide sodium salt, Pierce Chemicals•EDC: 1-Ethyl-3-(3-dimethy

Strany 29 - Specifications

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 6 PN 89-00002-00-150 Rev. A11. Incubate the suspension for 20 ± 2 minutes in the dark a

Strany 30 - Luminex XYP

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 7amines. If any of these agents exist in the protein preparation, remove them through dialysis

Strany 31 - Uninterruptible power

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 8 PN 89-00002-00-150 Rev. A•SDS:Sodium Dodecyl Sulfate (0.1% w/v)Preparation 1. Allow a

Strany 32 - Overview

xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 916. Centrifuge the xMAP microspheres at 8,000 × g or higher for 1 minute.17. Remove the supern

Strany 33 - Ventilation Filter

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 10 PN 89-00002-00-150 Rev. A• Micropipetters (1 µl - 1000 µl)• Microcentrifuge•Timer•Ro

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xMAP Technology ProtocolsPN 89-00002-00-150 Rev. A 9 - 119. Add 500 µl of BLOCKING/STORAGE BUFFER to the microspheres and vortex. Centrifuge the tube

Strany 35 - See Figure 3-4

PN 89-00002-00-150 Rev. A 1 - 1IntroductionThe Luminex® 200™ system is a compact analysis unit consisting of a Luminex analyzer, the Luminex XY Platfo

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology9 - 12 PN 89-00002-00-150 Rev. A

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PN 89-00002-00-150 Rev. A A - 1Glossaryaggregated microspheresTwo or more xMAP microspheres that have adhered together.Alexa 532 Fluorochrome used for

Strany 38 - Luminex XYP Heater

A - 2 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technologycalibrators xMAP microspheres used to normalize the settings

Strany 39 - Installation

PN 89-00002-00-150 Rev. A A - 3xMAP Technology emission spectrumemission spectrum Wavelength range that an excited fluorochrome emits when its electro

Strany 40 -  To set up the PC:

A - 4 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologyxMAP microsphere set Luminex multi-analyte microspheres cont

Strany 41 - Software Setup

PN 89-00002-00-150 Rev. A A - 5xMAP Technology reporterreporter A molecule (or combination of molecules) with a specific range of excitation and emiss

Strany 42 - Sample Probe

A - 6 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

Strany 43 - Note: To avoid adjusting the

PN 89-00002-00-150 Rev. A B - 1Product NumbersNote: These part numbers are subject to change without notification.HardwareBAir filter (accessed throug

Strany 44 - Installing Software

B - 2 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologySoftwareReagentsPower cord CN-P002-01 (Australia)CN-P003-01

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PN 89-00002-00-150 Rev. A B - 3xMAP TechnologyTrainingxMAP development microspheres (carboxylated)1, 4, and 16 mLL100-CDEV1-[size code of 01, 04, or 1

Strany 46

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology1 - 2 PN 89-00002-00-150 Rev. Aits own subset, based on its fluorescent signature. In addit

Strany 47 - System Startup

B - 4 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

Strany 48 - Channel Calibration Lots

PN 89-00002-00-150 Rev. A C - 1Luminex Sheath Delivery SystemOverview Use this appendix to install, use, and maintain the Luminex Sheath Delivery Syst

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Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 2 PN 89-00002-00-150 Rev. AWARRANTY RIGHTS PROVIDED BY LUMINEX AND/OR ITS AUTHORIZED PA

Strany 50 - Add New Reporter

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-150 Rev. A C - 3Sheath Delivery System SetupWhen adjusting the Luminex 200 regulator, tur

Strany 51 - Calibrate the Luminex

Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 4 PN 89-00002-00-150 Rev. A• Connect the other end of the air line to the air connector

Strany 52

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-150 Rev. A C - 510. In the Luminex Data Collector Main window, click Prime. During this p

Strany 53 - Calibration Report

Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 6 PN 89-00002-00-150 Rev. AMaintenance and CleaningPerform proper maintenance and clean

Strany 54 - Figure 5-10. Calibration Log

xMAP Technology Luminex Sheath Delivery SystemPN 89-00002-00-150 Rev. A C - 7Filter. If the filter attached to the sheath intake line becomes clogged

Strany 55 - Analyzer menu

Luminex 200 User Manual for LDS Version 1.7 xMAP TechnologyC - 8 PN 89-00002-00-150 Rev. ALabels The following labels are attached to the back of the

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PN 89-00002-00-150 Rev. A Index-1Numerics100 region bead map 6-225 region bead map 6-250 region bead map 6-2Aaccess doors 3-8accuracy/precisionspecifi

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xMAP Technology IntroductionPN 89-00002-00-150 Rev. A 1 - 3container and effectively removes residual sample within the tubing, valves, and probe. The

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Index-2 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technologycaution 2-6cautions 2-2, 2-5, 2-8CE mark 2-4centrifugation

Strany 59 - Data Acquisition

PN 89-00002-00-150 Rev. A Index-3xMAP Technology Indexheater temperature 6-22height adjustment locking screw 4-4high voltage 6-22, 6-24histogram 6-17b

Strany 60 - Figure 6-2. 100 Region MAP

Index-4 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technologyspecifications 3-4options dialog box 6-1PPC specifications

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PN 89-00002-00-150 Rev. A Index-5xMAP Technology Indexaccuracy/precision 3-2capacity 3-3classification laser 3-4electronics 3-4fluidics 3-4Luminex 100

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Index-6 PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP Technology

Strany 63 - °C and 60°C (95°F

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology1 - 4 PN 89-00002-00-150 Rev. AFluorescence CompensationFluorescent spillover occurs when a

Strany 64 - Template

xMAP Technology IntroductionPN 89-00002-00-150 Rev. A 1 - 5from the main menu bar, from the toolbar, and from menus that appear when you right-click a

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology1 - 6 PN 89-00002-00-150 Rev. A

Strany 66 - Existing Session

PN 89-00002-00-150 Rev. A 2 - 1SafetyYour safety is important. Please do not perform procedures on your Luminex 200 system that are not specifically c

Strany 67 - Open an Existing Session

© LUMINEX CORPORATION, 1998 - 2005. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into a

Strany 68 - Multiplex

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 2 PN 89-00002-00-150 Rev. ASafety and Regulatory SymbolsThese symbols describe warnings

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xMAP Technology SafetyPN 89-00002-00-150 Rev. A 2 - 3Danger: This message is used in cases where significant risk of serious injury or death is presen

Strany 70 - Main Control and Session

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 4 PN 89-00002-00-150 Rev. AFigure 2-2. European Safety Requirements LabelThe Luminex a

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xMAP Technology SafetyPN 89-00002-00-150 Rev. A 2 - 5Fluidics The Luminex analyzer contains fluidics. In the event of a fluid leak, turn off all power

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 6 PN 89-00002-00-150 Rev. AFigure 2-6. Laser Caution LabelDo not remove the analyzer c

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xMAP Technology SafetyPN 89-00002-00-150 Rev. A 2 - 7Figure 2-8. Avoid Exposure LabelMechanicalBiologicalCaution: Use of controls or adjustments or p

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology2 - 8 PN 89-00002-00-150 Rev. AHeatBlue Indicator LightThe blue light above the sample arm

Strany 75 -  To autoscale the Y axis:

PN 89-00002-00-150 Rev. A 3 - 1System OverviewHardware The Luminex 200 for LDS Version 1.7 system includes the following hardware:• Luminex analyzer•

Strany 76 -  To set a gate:

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 2 PN 89-00002-00-150 Rev. ARequired Laboratory ReagentsThe following items are necessar

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xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 3Sensitivity • Detect 1000 fluorochromes phycoerythrin (PE) per xMAP microsphere• Reporte

Strany 78 - Bar Graph/Dot Plot

Standard Terms and Conditions For Use of ProductBy opening the packaging containing this product ("Product") or by using such Product in any

Strany 79

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 4 PN 89-00002-00-150 Rev. A• AC inlet fuse: 3 Amp, 250 V~, fast actingOptics • Reporter

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xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 5Luminex SD System General• Ambient temperature: 15°C to 30°C (59° to 86°F)• Humidity: 20

Strany 81

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 6 PN 89-00002-00-150 Rev. Aand be UL listed, CSA certified, CE marked for nondomestic u

Strany 82 - Status Bar

xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 7Luminex XYPInstrumentVentilation FilterThe XYP instrument ventilation filter cleans the

Strany 83 - Run an Entire Plate of

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 8 PN 89-00002-00-150 Rev. AFluidics Luminex XYP Sample Probe: A stainless steel sampl

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xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 9Figure 3-3 Front View of Luminex analyzer Showing Access DoorsAir filter and access doo

Strany 85 - Interrupt a Run

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 10 PN 89-00002-00-150 Rev. ASyringe: The syringe delivers a sample from the 96 well mi

Strany 86 -  To print reports:

xMAP Technology System OverviewPN 89-00002-00-150 Rev. A 3 - 11Figure 3-7 Left Side of Analyzer - Air and Fluid ConnectorsSheath fluid container (not

Strany 87 - Generic/Text Only

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology3 - 12 PN 89-00002-00-150 Rev. AOptical The optical system contains the optics assembly and

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PN 89-00002-00-150 Rev. A 4 - 1InstallationLuminex 200 System InstallationEnsure that the facility complies with all system and safety requirements. I

Strany 89 - Maintenance and Cleaning

strict compliance with the United States Food and Drug Administration and all applicable domestic and international laws and regulations, now and here

Strany 90

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 2 PN 89-00002-00-150 Rev. A5. Place the Luminex analyzer on top of the Luminex XYP. Do

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xMAP Technology InstallationPN 89-00002-00-150 Rev. A 4 - 3Luminex 200 Software SetupAfter performing the Luminex 200 system assembly, ensure that the

Strany 92 - Surfaces

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 4 PN 89-00002-00-150 Rev. AAdjusting the Sample Probe HeightYou must adjust the sample

Strany 93 - Replace Air Intake

xMAP Technology InstallationPN 89-00002-00-150 Rev. A 4 - 5For a filter bottom plate—stack three of the larger (5.08 mm diameter) alignment discs toge

Strany 94 - Warning: The syringe arm does

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 6 PN 89-00002-00-150 Rev. AInstalling Software and FirmwareThe Luminex 200 system arriv

Strany 95 - Change Sheath

xMAP Technology InstallationPN 89-00002-00-150 Rev. A 4 - 7Time, Monday through Friday. Users outside of the U.S. and Canada can call us at +1 512-381

Strany 96 - Quick-disconnect

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology4 - 8 PN 89-00002-00-150 Rev. A

Strany 97 - Probe Vertical

PN 89-00002-00-150 Rev. A 5 - 1 System StartupThis chapter describes how to start up your system. The topics included are:• Luminex Main Window• Calib

Strany 98

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 2 PN 89-00002-00-150 Rev. AThe Luminex Main window has six components: Title bar, Menu

Strany 99 - Shipment

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 3 To add a new lot of xMAP classification calibration microspheres:1. Click Calibrate. T

Strany 100

End-User License Agreement (EULA) for Luminex® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an in

Strany 101

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 4 PN 89-00002-00-150 Rev. AAdd New Reporter Channel Calibration LotsBefore you perform

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xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 5Calibrate the Luminex AnalyzerIn addition to calibrating the analyzer after installation,

Strany 103 - Troubleshooting

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 6 PN 89-00002-00-150 Rev. A7. Fill the reservoir with a solution of 70% isopropanol or

Strany 104 - Problems

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 7The progress bar shows the calibration progress, which should take less than two minutes.

Strany 105

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 8 PN 89-00002-00-150 Rev. AFigure 5-9. Print Dialog Box3. Check the box in front of th

Strany 106

xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 9more) in the gain value for a channel from one calibration to the next, the difference ma

Strany 107

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 10 PN 89-00002-00-150 Rev. Aif a Luminex XYP instrument is added to the system. See pag

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xMAP Technology System StartupPN 89-00002-00-150 Rev. A 5 - 11recommend that you do not cancel warmup. Your test results will be accurate only if the

Strany 109 - Calibration

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology5 - 12 PN 89-00002-00-150 Rev. A

Strany 110

PN 89-00002-00-150 Rev. A 6 - 1Data AcquisitionThe Luminex analyzer uses Luminex Data Collector software to acquire data. This chapter describes the f

Strany 111 - Acquisition

be accessed through use of the SOFTWARE PRODUCT is the property of the respective content owner and may be protected by applicable copyright or other

Strany 112

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 2 PN 89-00002-00-150 Rev. ASound effects: Enables sounds during system operation.Clear

Strany 113 - Irregularities

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 3Acquisition tab On this tab you can select the default folder for your session files. I

Strany 114

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 4 PN 89-00002-00-150 Rev. AShow APD Voltages. This is a Field Service option. Do not se

Strany 115 - Error Messages

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 5To use the heater, check Enabled in the Heater area. Use the Set Point control to selec

Strany 116 - Verification

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 6 PN 89-00002-00-150 Rev. ACreate a Session Using Default Settings To create a sessio

Strany 117

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 72. Select the template that you want to use for this session and click OK. If the templ

Strany 118

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 8 PN 89-00002-00-150 Rev. AOpen and Add to an Existing Session To open an existing ses

Strany 119 - Protocols

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 93. Use the settings shown or modify them, then highlight the first vacant sample.4. Cli

Strany 120

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 10 PN 89-00002-00-150 Rev. A2. Make desired setting changes. See “Session Settings” on

Strany 121

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 11Open. Opens an existing sessionClose Session. Closes the current session. Prompts you

Strany 122 - Equipment •Vortex

PN 89-00002-00-150 Rev. A iContentsIntroduction 1-1Principle of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1Basi

Strany 123

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 12 PN 89-00002-00-150 Rev. AHelp Contents. Opens the online help.Search for Help On. Op

Strany 124

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 13Clear All: Clears the screen of all data and graphs. This function deletes all associ

Strany 125

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 14 PN 89-00002-00-150 Rev. ARight-Click Menu Other commands appear on the right-click m

Strany 126

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 15We recommend using at least 100 events per microsphere set. This guarantees that each

Strany 127 - LumAvidin-Modified

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 16 PN 89-00002-00-150 Rev. ABead Set tab On the Bead Set tab you can select and name th

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xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 17Histogram The histogram is located in the lower-left corner of the screen. The histogr

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 18 PN 89-00002-00-150 Rev. A2. Click X axis, then click on the desired parameter name.I

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xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 19Use the Show Bead options to set the histogram to show events for only one bead set, a

Strany 131 - Glossary

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 20 PN 89-00002-00-150 Rev. Afor later assays or sessions. By increasing this voltage se

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xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 21density is indicated by contrasting colors. A decaying dot plot shows the 100 most rec

Strany 133

ii PN 89-00002-00-150 Rev. ALuminex 200 User Manual for LDS Version 1.7 xMAP TechnologyLuminex XYP Specifications. . . . . . . . . . . . . . . . . . .

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Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 22 PN 89-00002-00-150 Rev. Athe beads are hitting with respect to their region. The bea

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xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 23The message at the top reflects the current status of the Luminex analyzer.Ready: Indi

Strany 136

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 24 PN 89-00002-00-150 Rev. AThe system monitor section also shows information about tem

Strany 137 - Product Numbers

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 255. In the results table of the new session, highlight the sample where you want to beg

Strany 138 - Reagents

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 26 PN 89-00002-00-150 Rev. A9. Remove the microtiter plate from the plate holder.10. Cl

Strany 139 - Training

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 279. Remove the microtiter plate from the plate holder.10. Click Retract from the toolba

Strany 140

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 28 PN 89-00002-00-150 Rev. A To interrupt sample acquisition between sample runs:1. Cl

Strany 141 - Specifications

xMAP Technology Data AcquisitionPN 89-00002-00-150 Rev. A 6 - 296. In the Manufacturers List, click Generic. In Printers list, click Generic/Text Only

Strany 142

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology6 - 30 PN 89-00002-00-150 Rev. A

Strany 143 - System Setup

PN 89-00002-00-150 Rev. A 7 - 1Maintenance and CleaningProper maintenance and cleaning of the Luminex analyzer should be performed in order to preserv

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PN 89-00002-00-150 Rev. A iiixMAP Technology ContentsDelete a Template. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-10Multiplex

Strany 145 - Delivery System

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 2 PN 89-00002-00-150 Rev. AStartup  Perform the entire startup procedure on a daily ba

Strany 146

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 3Check Fluid Levels Check the sheath and waste fluids daily, more often if you r

Strany 147

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 4 PN 89-00002-00-150 Rev. AClean Accessible Surfaces Clean the exterior surfaces of th

Strany 148

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 5Replace Air Intake FiltersYou should replace the filters on the Luminex analyze

Strany 149 - Numerics

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 6 PN 89-00002-00-150 Rev. A Figure 7-2. Back of Luminex XYP, Removing Air Filter3. Rep

Strany 150

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 71. Syringe Seal 2. SyringeFigure 7-3. Front of Luminex 200 Analyzer,

Strany 151

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 8 PN 89-00002-00-150 Rev. A3. Connect the new sheath filter, matching up the color-code

Strany 152

xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 9Change Fuses  To replace fuses on the Luminex analyzer or the Luminex XYP ins

Strany 153

Luminex 200 User Manual for LDS Version 1.7 xMAP Technology7 - 10 PN 89-00002-00-150 Rev. AStoring the Analyzer  To prepare the system for storage:1.

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xMAP Technology Maintenance and CleaningPN 89-00002-00-150 Rev. A 7 - 11Decontaminating the Luminex Analyzer for Return ShipmentLuminex Technical Supp

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